Working with Chromatograms

All operations for processing single chromatograms are performed in the Chromatogram window. This window displays the integrated chromatogram of the current sample. The peak areas are determined and integrated automatically, based on the Detection Parameters.

If the representation and/or evaluation of single samples do not correspond to the expected result, you can select another chromatogram section to specifically change the peak delimiters and the baseline manually. It is also possible to display more chromatograms for comparison. In addition, you can assign the peaks manually and define a baseline for the entire chromatogram (averaged baseline). You can also apply a data filter to smooth the chromatogram and to improve the reproducibility of the peak baselines.

For more information, refer to:

 Using Keyboard Shortcuts in the Chromatogram

 Manual Re-Integration

 Manual Peak Assignment

 Comparing Chromatograms

 Performing Data Smoothing

You can also generate additional channels after data acquisition. Refer to:

 Copying a Channel

 Combining Channels via Arithmetic Operations

Due to the increased noise, Dionex recommends performing background subtraction for mass spectra. This is also possible for UV channels. In both cases, determine background subtraction in the chromatogram (see  Subtracting Background Spectra).

In addition, you can  Display Peak Information in a Separate Area.

To graphically move the retention window, refer to  Moving a Retention Window Graphically.

If you want to change the detection parameters, for example, because peaks that are too small or unimportant are integrated or because the baseline does not meet the requirements, you can perform these changes graphically in the chromatogram; see  Defining Detection Parameters Graphically.

You can define the detection parameters according to your requirements. All detection parameters are available in the QNT Editor on the Detection tab page (see Integrating Chromatograms and Identifying Peaks  Defining Detection Parameters; for information about the editor, refer to Data Representation and Reprocessing  The QNT Editor).

For more information about how to display a spectra plot, an I-t plot, or a Report, refer to

 Displaying and Using UV Spectra

 Displaying and Using I-t Plots

 Creating and Using Report Tables